Composite

Part:BBa_K3396003:Design

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-26)


GFPnano-FKBP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35


Design Notes

To realize TRIM21 can still connect with GFPnano, we make the GFPnano tagged with FKBP, which can connect with FRB induced by rapamycin.


Source

The sequence of GFP-nano we use came from a patent. We choose FKBP through surveying on literature.

References